\u201cFocused Ion Beam Scanning Electron Microscopes for Volume-EM and Cryo-Microscopy<\/strong>\u201d<\/h2>\n<\/blockquote>\n\n\n\n _<\/p>\n\n\n\n
Date: <\/h2>\n\n\n\n
3:30 PM – 5:00 PM Tuesday, September 19th<\/sup>, 2023 (JST), <\/p>\n\n\n\nLocation: Hybrid<\/strong> (CryoEM-Lab KEK and Zoom)<\/p>\n\n\n\n<\/p>\n\n\n\n
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Speaker<\/h2>\n\n\n\n![\"\"](\"https:\/\/pf-form.kek.jp\/sbrc\/wp-content\/uploads\/sites\/39\/2023\/08\/AlexRigort.jpg\")
<\/figure>\n<\/div>\n\n\nDr. Alexander Rigort<\/h2>\n\n\n\n
Thermo Fisher Scientific, Munich, Germany<\/p>\n\n\n\n
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\n\n\n\nAbstract<\/h2>\n\n\n\n
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FIB\/SEM microscopes have proven to be indispensable tools for cryo-electron microscopy and volumetric imaging of biological specimens. These devices can be used to analyze samples at room temperature and under cryo-conditions. Equipped with automation software for both data acquisition and electron transparent lamella fabrication, FIB\/SEM microscopes offer a wide range of applications and multiple workflows for obtaining volumetric information. The imaging capabilities include high-resolution serial imaging with FIB\/SEM tomography (slice & view), millimeter-scale analysis with the spin mill method, array tomography and correlative microscopy with the iFLM system. Additionally, Plasma-FIB systems allow the use of up to four different ion species, which can be used to optimize milling and imaging for both cryogenic and resin-embedded samples. In this lecture, I will provide an overview of the current state of developments, applications, and the related FIB\/SEM product range.<\/p>\n\n\n\n
Profile<\/strong>:<\/p>\n\n\n\nDr. Alex Rigort<\/strong> works in product marketing and development for Thermo Fisher Scientific and holds a guest researcher position at the Max Planck Institute of Biochemistry in Martinsried. He has pioneered the use of cryo-focused ion beam instruments for cryo-tomography at the Max Planck Institute of Biochemistry and has long-standing experience in cryo-electron microscopy.<\/p>\n\n\n\nLink to Publications: Dr. Alexander Rigort<\/a> (Google Scholar)<\/p>\n\n\n\nMore about the author: https:\/\/analyticalscience.wiley.com\/do\/10.1002\/was.0002057028\/<\/a><\/p>\n\n\n\n
\n\n\n\nClick here to apply<\/a><\/h2>\n\n\n\n<\/a>\n\n\n\n\n\n<\/p>\n","protected":false},"excerpt":{"rendered":"
\u201cFocused Ion Beam Scanning Electron Microscopes for Volume-EM and Cryo-Microscopy\u201d _ Date: 3:30 PM – 5:0 […]<\/p>\n","protected":false},"author":26,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-1564","post","type-post","status-publish","format-standard","hentry","category-kek-cryoem"],"_links":{"self":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts\/1564","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/users\/26"}],"replies":[{"embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/comments?post=1564"}],"version-history":[{"count":12,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts\/1564\/revisions"}],"predecessor-version":[{"id":1585,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts\/1564\/revisions\/1585"}],"wp:attachment":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/media?parent=1564"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/categories?post=1564"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/tags?post=1564"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Location: Hybrid<\/strong> (CryoEM-Lab KEK and Zoom)<\/p>\n\n\n\n <\/p>\n\n\n\n \u3000\u3000\u3000\u3000<\/p>\n\n\n\n Thermo Fisher Scientific, Munich, Germany<\/p>\n\n\n\n <\/p>\n\n\n\n <\/p>\n\n\n\n <\/p>\n\n\n\n <\/p>\n\n\n\n <\/p>\n\n\n\n <\/p>\n\n\n\n FIB\/SEM microscopes have proven to be indispensable tools for cryo-electron microscopy and volumetric imaging of biological specimens. These devices can be used to analyze samples at room temperature and under cryo-conditions. Equipped with automation software for both data acquisition and electron transparent lamella fabrication, FIB\/SEM microscopes offer a wide range of applications and multiple workflows for obtaining volumetric information. The imaging capabilities include high-resolution serial imaging with FIB\/SEM tomography (slice & view), millimeter-scale analysis with the spin mill method, array tomography and correlative microscopy with the iFLM system. Additionally, Plasma-FIB systems allow the use of up to four different ion species, which can be used to optimize milling and imaging for both cryogenic and resin-embedded samples. In this lecture, I will provide an overview of the current state of developments, applications, and the related FIB\/SEM product range.<\/p>\n\n\n\n Profile<\/strong>:<\/p>\n\n\n\n Dr. Alex Rigort<\/strong> works in product marketing and development for Thermo Fisher Scientific and holds a guest researcher position at the Max Planck Institute of Biochemistry in Martinsried. He has pioneered the use of cryo-focused ion beam instruments for cryo-tomography at the Max Planck Institute of Biochemistry and has long-standing experience in cryo-electron microscopy.<\/p>\n\n\n\n Link to Publications: Dr. Alexander Rigort<\/a> (Google Scholar)<\/p>\n\n\n\n More about the author: https:\/\/analyticalscience.wiley.com\/do\/10.1002\/was.0002057028\/<\/a><\/p>\n\n\n\n <\/p>\n","protected":false},"excerpt":{"rendered":" \u201cFocused Ion Beam Scanning Electron Microscopes for Volume-EM and Cryo-Microscopy\u201d _ Date: 3:30 PM – 5:0 […]<\/p>\n","protected":false},"author":26,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-1564","post","type-post","status-publish","format-standard","hentry","category-kek-cryoem"],"_links":{"self":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts\/1564","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/users\/26"}],"replies":[{"embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/comments?post=1564"}],"version-history":[{"count":12,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts\/1564\/revisions"}],"predecessor-version":[{"id":1585,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts\/1564\/revisions\/1585"}],"wp:attachment":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/media?parent=1564"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/categories?post=1564"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/tags?post=1564"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}Speaker<\/h2>\n\n\n
<\/figure>\n<\/div>\n\n\nDr. Alexander Rigort<\/h2>\n\n\n\n
\n\n\n\nAbstract<\/h2>\n\n\n\n
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