{"id":1805,"date":"2023-11-28T10:03:00","date_gmt":"2023-11-28T01:03:00","guid":{"rendered":"https:\/\/pf-form.kek.jp\/sbrc\/?p=1805"},"modified":"2023-11-28T10:03:00","modified_gmt":"2023-11-28T01:03:00","slug":"seminar17","status":"publish","type":"post","link":"https:\/\/pf-form.kek.jp\/sbrc\/seminar17\/","title":{"rendered":"SBRC International Cryo-EM Seminar No.17"},"content":{"rendered":"\n

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\u201cStructure determination of RNA-dependent RNA Polymerase 2 (RDR2) by cryo-EM and its implications for double-stranded RNA synthesis in gene silencing\u201d<\/h2>\n<\/blockquote>\n\n\n\n

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Date: <\/h2>\n\n\n\n

4:00 PM – 5:30 PM Tuesday, December 19th<\/sup>, 2023 (JST), <\/p>\n\n\n\n

Location: Hybrid<\/strong> (CryoEM-Lab KEK and Zoom)<\/p>\n\n\n\n

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\u3000\u3000\u3000\u3000<\/p>\n\n\n\n

Speaker<\/h2>\n\n\n
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Yuichiro H. Takagi, Ph.D.<\/h2>\n\n\n\n

Associate Professor
Scientific advisor for cryo-EM 
Department of Biochemistry and Molecular Biology
Indiana University School of Medicine<\/p>\n\n\n\n

Takagi Lab<\/a><\/p>\n\n\n\n

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Abstract<\/h2>\n\n\n\n

RNA-dependent RNA polymerases (RdRP) play critical roles in RNA-mediated gene silencing. In Arabidopsis thaliana<\/em>, RNA-Dependent RNA Polymerase 2 (RDR2) generates double-stranded RNAs (dsRNAs) from a single stranded RNA template generated by DNA-dependent nuclear RNA Polymerase IV (Pol IV). Resulting dsRNAs are then processed into short-interfering RNAs that guide RNA-directed DNA methylation and transcriptional gene silencing. We determined the structure of RDR2 at 3.1 \u00c5 resolution by single-particle cryo-electron microscopy. The structure suggests its implications for double-stranded RNA synthesis in gene silencing.  The seminar will also touch on several structure examples determined by Takagi lab using cryo-EM including Mediator CDK8 module from the yeast Saccharomyces cerevisiae<\/em>,  human asparagine synthetase, and multicopper oxidase from Marinithermus hydrothermalis<\/em>.<\/p>\n\n\n\n

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Click here to apply<\/a><\/h2>\n\n\n\n<\/a>\n\n\n\n\n\n

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\u201cStructure determination of RNA-dependent RNA Polymerase 2 (RDR2) by cryo-EM and its implications for double-s […]<\/p>\n","protected":false},"author":26,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-1805","post","type-post","status-publish","format-standard","hentry","category-kek-cryoem"],"_links":{"self":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts\/1805","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/users\/26"}],"replies":[{"embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/comments?post=1805"}],"version-history":[{"count":7,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts\/1805\/revisions"}],"predecessor-version":[{"id":1829,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts\/1805\/revisions\/1829"}],"wp:attachment":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/media?parent=1805"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/categories?post=1805"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/tags?post=1805"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}