{"id":3482,"date":"2026-07-06T08:59:22","date_gmt":"2026-07-05T23:59:22","guid":{"rendered":"https:\/\/pf-form.kek.jp\/sbrc\/?p=3482"},"modified":"2026-07-06T14:33:56","modified_gmt":"2026-07-06T05:33:56","slug":"seminar35","status":"publish","type":"post","link":"https:\/\/pf-form.kek.jp\/sbrc\/seminar35\/","title":{"rendered":"SBRC International Cryo-EM Seminar No.35"},"content":{"rendered":"\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\">\n<h2 class=\"wp-block-heading\">\u201cVisualizing Molecular Machines in Motion through Cryo-EM, Cryo-ET, and Deep Learning\u201d<\/h2>\n<\/blockquote>\n\n\n\n<p class=\"wp-block-paragraph\"> _<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Date and Time<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\" style=\"font-size:25px\">9:30 AM &#8211; 11:00 AM Tuesday, July 28, 2026<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Location<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\" style=\"font-size:25px\">Online (Zoom)<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\" \/>\n\n\n\n<h2 class=\"wp-block-heading\" style=\"font-size:30px\">Speaker<\/h2>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"275\" height=\"363\" src=\"http:\/\/pf-form.kek.jp\/sbrc\/wp-content\/uploads\/sites\/39\/2026\/07\/Dr.-Joseph-H.-Davis-PhD.jpg\" alt=\"\" class=\"wp-image-3501\" style=\"width:202px;height:auto\" srcset=\"https:\/\/pf-form.kek.jp\/sbrc\/wp-content\/uploads\/sites\/39\/2026\/07\/Dr.-Joseph-H.-Davis-PhD.jpg 275w, https:\/\/pf-form.kek.jp\/sbrc\/wp-content\/uploads\/sites\/39\/2026\/07\/Dr.-Joseph-H.-Davis-PhD-227x300.jpg 227w\" sizes=\"auto, (max-width: 275px) 100vw, 275px\" \/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" style=\"font-size:28px\">Dr. Joseph H. Davis, PhD<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Whitehead Associate Professor of Biology, Massachusetts Institute of Technology (MIT), US. Associate Member, Broad Institute of MIT and Harvard, US<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u3000<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Abstract<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Macromolecular machines undergo massive compositional and conformational rearrangements during their lifecycles to maintain cellular homeostasis. While cryo-EM and cryo-ET have revolutionized our ability to determine these structures, reconstructing highly heterogeneous ensembles and continuous trajectories of molecular motion directly from raw, low-signal experimental data remains a formidable biochemical and computational challenge. In this talk, I will describe our lab\u2019s ongoing efforts to bridge the gap between experimental structural biology and advanced machine learning to resolve these complex structural landscapes. First, I will present cryoPRISM (purification-free ribosome imaging from subcellular mixtures), a rapid ex vivo workflow that bypasses extensive biochemical purification to capture biological complexes in near-native environments. Second, I will discuss our development of deep learning architectures, such as cryoDRGN and its extension tomoDRGN, which leverage variational autoencoders (VAEs) to map single-particle images or sub-tomograms to continuous, low-dimensional latent spaces. These computational tools enable the unsupervised reconstruction of continuous conformational changes and compositional heterogeneity directly from low-signal-to-noise datasets, paving the way for a deeper, quantitative understanding of cellular machinery in action.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>Profile<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">2024\u2013Present: Whitehead Associate Professor of Biology, MIT &amp; Associate Member, Broad Institute of MIT &amp; Harvard<br>2017\u20132024: Assistant Professor of Biology, MIT<br>2012\u20132017: Jane Coffin Childs Postdoctoral Fellow and NIH K99 Fellow, the Scripps Research Institute<br>2010\u20132012: Senior Scientist and First Employee, Ginkgo BioWorks<br>2005\u20132010: Ph.D. in Biology, Massachusetts Institute of Technology<br>2000\u20132003: B.S. in Biological Engineering and B.A. in Computer Science, Univ. of California, Berkeley<\/p>\n\n\n\n<hr class=\"wp-block-separator has-css-opacity is-style-dots\" \/>\n\n\n\n<h2 class=\"wp-block-heading has-text-align-center has-white-color has-cyan-bluish-gray-background-color has-text-color has-background\"><a href=\"https:\/\/pf-form.kek.jp\/sbrc\/registration_for_internationalseminar\/\" data-type=\"link\" data-id=\"https:\/\/pf-form.kek.jp\/sbrc\/registration_for_international_seminar\/\" target=\"_blank\" rel=\"noreferrer noopener\">Click here to apply<\/a><\/h2>\n","protected":false},"excerpt":{"rendered":"<p>\u201cVisualizing Molecular Machines in Motion through Cryo-EM, Cryo-ET, and Deep Learning\u201d _ Date and Time 9:30 AM [&hellip;]<\/p>\n","protected":false},"author":26,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-3482","post","type-post","status-publish","format-standard","hentry","category-kek-cryoem"],"_links":{"self":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts\/3482","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/users\/26"}],"replies":[{"embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/comments?post=3482"}],"version-history":[{"count":4,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts\/3482\/revisions"}],"predecessor-version":[{"id":3508,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/posts\/3482\/revisions\/3508"}],"wp:attachment":[{"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/media?parent=3482"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/categories?post=3482"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pf-form.kek.jp\/sbrc\/wp-json\/wp\/v2\/tags?post=3482"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}