“Structural Studies of Viral Macromolecular Complexes by Cryo-Electron Microscopy ”
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Date and Time
4:00 PM – 5:30 PM Friday, October 3th, 2025
Location
Onsite（CryoEM building）
Speakers: Yoko Fujita-Fujiharu
Postdoc
Max Planck Institute of Biochemistry
German,
Abstract
Understanding the architecture of viral macromolecular complexes is essential for elucidating the mechanisms of viral assembly and replication. While traditional structural biology often focuses on purified proteins in isolation, cryo-electron microscopy (cryo-EM) has the potential to analyze three-dimensional structures in more biologically relevant contexts.
In this talk, I will present some results from a study integrating multi-scale structural information of Ebola virus-like particles (VLPs). By combining in situ single-particle analysis and cryo-electron tomography, we resolved the structures of the viral matrix protein VP40 and the glycoprotein GP within intact VLPs. This multi-scale cryo-EM approach provides structural insights ranging from angstrom-level atomic detail to nanometer-scale organization, offering a more comprehensive understanding of viral assembly and function.